Significance of m6A regulatory factor in gene expression and immune function of osteoarthritis

One of the most prevalent posttranscriptional modifications of eukaryotic mRNA is the RNA N6-methyladenosine (m 6 A) regulator, which plays a significant role in various illnesses. The involvement of m 6 A regulators in osteoarthritis (OA) is not fully known. By comparing nonosteoarthritic and osteoarthritic patients, 26 important m 6 A regulators were identified from the gene expression omnibus GSE48556 dataset. Seven candidate m 6 A regulators (IGFBP3, WTAP, IGFBP1, HNRNPC, RBM15B, YTHDC1, and METTL3) were screened using a random forest model to assess the likelihood of OA. A column line graph model founded on seven m 6 A modulator candidates was created. According to decision curve analysis, patients might profit from the column line graph model. Based on chosen relevant m 6 A modifiers, a consensus clustering approach was utilized to categorize OA into two m 6 A categories (group A and group B). To measure the m 6 A pattern, a principal component analysis technique was created to generate the m 6 A score for every sample. Cluster A patients exhibited more excellent m 6 A scores than cluster B patients. Furthermore, we discovered that patients with lower and higher m 6 A scores had varied immunological responses using the m 6 A type. At last, m 6 A regulators contribute significantly to the progression of OA. Our research on m 6 A patterns might help to guide further OA immunotherapeutic techniques.