Optimizing neural stem cell transfection with NeuroMag

Optimizing neural stem cell transfection with NeuroMag

Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection...

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Veröffentlicht in:BioTechniques 2017-01, Vol.62 (1), p.xii
Hauptverfasser: Sapet, Cédric, Laurent, Nicolas, de Chevigny, Antoine, Gourrierec, Loïc Le, Bertosio, Elodie, Zelphati, Olivier, Béclin, Christophe
Format: Artikel
Sprache:eng
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Zusammenfassung:Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation.
ISSN:0736-6205
1940-9818
DOI:10.2144/000114500