siRNA knockdown validation 101: Incorporating negative controls in antibody research [version 1; peer review: 3 approved]

siRNA knockdown validation 101: Incorporating negative controls in antibody research [version 1; peer review: 3 approved]

More antibody validation protocols would identify non-specific reagents - a major source of irreproducible research - with the inclusion of negative controls. This article presents an overview of one such method: siRNA knockdown. The authors outline a general protocol, the knockdown mechanism, and t...

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Veröffentlicht in:F1000 research 2016, Vol.5, p.308-308
Hauptverfasser: Olds, Will, Li, Jason
Format: Artikel
Sprache:eng
Schlagworte:
Antigen Processing & Recognition
Opinion
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Zusammenfassung:More antibody validation protocols would identify non-specific reagents - a major source of irreproducible research - with the inclusion of negative controls. This article presents an overview of one such method: siRNA knockdown. The authors outline a general protocol, the knockdown mechanism, and tips for evaluating knockdown experiments.
ISSN:2046-1402
2046-1402
DOI:10.12688/f1000research.8159.1