De novo identification of bacterial antigens of a clinical isolate by combining use of proteosurfaceomics, secretomics, and BacScan technologies

Emerging infectious diseases pose a significant threat to both human and animal populations. Rapid identification of protective antigens from a clinical isolate and development of an antigen-matched vaccine is a golden strategy to prevent the spread of emerging novel pathogens. Here, we focused on , which poses a serious threat to the pig industry, and developed a general workflow by integrating proteosurfaceomics, secretomics, and BacScan technologies for the rapid identification of bacterial protective proteins from a clinical isolate. As a proof of concept, we identified 3 novel protective proteins of . Using the protective protein HBS1_14 and toxin proteins, we have developed a promising multivalent subunit vaccine against . We believe that our strategy can be applied to any bacterial pathogen and has the potential to significantly accelerate the development of antigen-matched vaccines to prevent the spread of an emerging novel bacterial pathogen.