Integrated regulatory network in Pseudomonas syringae reveals dynamics of virulence

Pseudomonas syringae, a Gram-negative plant pathogen, expresses multitudinous transcriptional regulators to control the type III secretion system (T3SS) and response to diverse environmental challenges. Although the mechanisms of virulence-associated regulators of P. syringae have been studied for decades, the overall crosstalk underlying these regulators is still elusive. Here, we identify five T3SS regulators (EnvZ-OmpR, CbrAB2, PhoPQ, PilRS, and MgrA), and find that the two-component systems EnvZ-OmpR and CbrAB2 negatively regulate the T3SS. To elucidate crosstalk between 16 virulence-associated regulators in P. syringae, we map an online intricate network called “PSRnet” (Pseudomonas syringae regulatory network) by combining the differentially expressed genes (DEGs) of these 16 regulators by RNA sequencing (RNA-seq) and their binding loci by chromatin immunoprecipitation sequencing (ChIP-seq). Consequently, we identify 238 and 153 functional genes involved in the T3SS and other virulence-related pathways in KB and MM media, respectively. Our results provide insights into the mechanism of plant infections caused by P. syringae. [Display omitted] •EnvZ-OmpR, CbrAB2, PhoPQ, PilRS, and MgrA regulate virulence•A Pseudomonas syringae regulatory network (PSRnet) is constructed•PSRnet reveals hundreds of functional genes involved in virulence-related pathways•An online PSRnet platform provides network analysis services for users Shao et al. map an intricate network called “PSRnet” (Pseudomonas syringae regulatory network) by combining the differentially expressed genes (DEGs) of 16 regulators and their binding loci. PSRnet includes hundreds of functional genes involved in the type III secretion system (T3SS) and other virulence-related pathways in different nutrition environments.