Evaluation of the effect of liposomal miR-143 on cell viability and anti-apoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers

Introdution: The present study was conducted to evaluate the effect of miR-143 on the expression of Bcl2 gene at the level of mRNA, cell cytotoxicity in the SaOs-2 cell line (Osteosarcoma) and Hum 63 (Human primary bone cells). Methods: In this experimental study, miR-143 was introduced into the SaOs-2 and Hum 63 cell by the lipofectam 2000 system. Then, the effects of miR-143 on cytotoxicity (72 hours) and expression of Bcl2 gene were measured by the MTT tests and Real Time Pcr protocols, respectively. Results: The results showed that the lipofectamine 2000 system had no toxicity in both Hum63 and SaOs2 cell lines by itself. SaOs2 cancer cells were more susceptible to miR-143 and Lipo / miR-143 compared to Hum63 cells. The highest reduction in SaOs2 cells was observed after the treatment with Lipo / miR-143 (p