Epigenomic profiling of isolated blood cell types reveals highly specific B cell smoking signatures and links to disease risk

Tobacco smoking alters the DNA methylation profiles of immune cells which may underpin some of the pathogenesis of smoking-associated diseases. To link smoking-driven epigenetic effects in specific immune cell types with disease risk, we isolated six leukocyte subtypes, CD14+ monocytes, CD15+ granul...

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Veröffentlicht in:	Clinical epigenetics 2023-05, Vol.15 (1), p.90-90, Article 90
Hauptverfasser:	Wang, Xuting, Campbell, Michelle R, Cho, Hye-Youn, Pittman, Gary S, Martos, Suzanne N, Bell, Douglas A
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Blood Blood cell types CD14 antigen CD19 antigen CD4 antigen CD56 antigen CD8 antigen Cell activation Cells CpG Islands Disease DNA Methylation Epigenesis, Genetic Epigenetics Epigenome-wide association study Epigenomics Genetic variation Genome-wide association studies Genome-Wide Association Study Genomes Humans Immune system Immunological memory Leukocytes Leukocytes (granulocytic) Linkage disequilibrium Lung diseases Lymphocytes B Lymphocytes T Memory B cell Memory cells Monocytes Natural killer cells Quantitative trait loci Respiratory function Single-nucleotide polymorphism Smoking Smoking - adverse effects Smoking - genetics Statistical significance Tobacco smoke Tobacco Smoking
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Zusammenfassung:	Tobacco smoking alters the DNA methylation profiles of immune cells which may underpin some of the pathogenesis of smoking-associated diseases. To link smoking-driven epigenetic effects in specific immune cell types with disease risk, we isolated six leukocyte subtypes, CD14+ monocytes, CD15+ granulocytes, CD19+ B cells, CD4+ T cells, CD8+ T cells, and CD56+ natural killer cells, from whole blood of 67 healthy adult smokers and 74 nonsmokers for epigenome-wide association study (EWAS) using Illumina 450k and EPIC methylation arrays. Numbers of smoking-associated differentially methylated sites (smCpGs) at genome-wide significance (p
ISSN:	1868-7083 1868-7083 1868-7075
DOI:	10.1186/s13148-023-01507-8