Silver Nanoparticles as an Effective Anti-Nanobacterial System towards Biofilm Forming Pseudomonas oryzihabitans

Silver nanoparticles have been considered a powerful antimicrobial agents recently especially after increasing incidence of diseases associated with biofilm and multi-drug resistant pathogens required to find a novel path to eradicate that challenge. The present study aims to evaluate the antibacterial activity of biosynthesized silver nanoparticles (AgNPs) using a cell-free extract of Enterobacter cloacae and chemo synthesis by sodium borohydride (NaBH4) on biofilm-forming Pseudomonas oryzihabitans. Antimicrobial effect of silver nanoparticles in both types and in combination with imipenem were evaluated by agar well diffusion method. The results revealed a good response to inhibit biofilm-forming Pseudomonas oryzihabitans growth by silver nanoparticles antibacterial activity in both types (biological and chemical) and in combination with imipenem; the antimicrobial effect was increased and enhanced. In the present study, it was found that the biological and chemical silver nanoparticles were considered a novel and decisive solution against biofilm and multi- drug resistance bacteria with a preference of biological silver nanoparticles.