Effect of different culture media on the growth and antifungal activity ofBacillus pumilus CCIBP-C5

Plantains and bananas (Musa spp.) are crops that require abundant fertilization and are affectedby numerous pests and diseases, including Black Sigatoka. Previous studies demonstrated thepotential of theBacillus pumilus strain CCIBP-C5 for thein vitro and greenhouse control ofPseudocercospora fijiensis Morelet and to stimulate the growth ofMusa spp. Several studiesare still required to develop a bioproduct based on this strain. For this reason, this researchaims to determine the effect of different culture media on the growth and antifungal activityofBacillus pumilus CCIBP-C5. Two culture media made with industrial by-products, in whichthe growth kinetics of the strain and the effect of culture filtrates (FC) on the growthinhibition ofPseudocercospora fijiensis we re evaluate d. The bacterial strain was able to g rowin both culture media and inhibition of fungal growth was observed in all FCs. The FC obtainedin Soy-Chitin Broth produced greater growth inhibition compared to the FC obtained in Soy- Molasses Broth. It is concluded that the Soy Broth-Chitin culture medium could be the mostindicated for the elaboration of a bioproduct fromB. pumilus CCIBP-C5