Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato

Genetic gain in potato is hampered by the heterozygous tetraploid genome of cultivated potato. Converting potato into a diploid inbred-line based F1-hybrid crop provides a promising route towards increased genetic gain. The introduction of a dominant S-locus inhibitor ( Sli ) gene into diploid potat...

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Veröffentlicht in:Nature communications 2021-07, Vol.12 (1), p.4141-4141, Article 4141
Hauptverfasser: Eggers, Ernst-Jan, van der Burgt, Ate, van Heusden, Sjaak A. W., de Vries, Michiel E., Visser, Richard G. F., Bachem, Christian W. B., Lindhout, Pim
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Sprache:eng
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Zusammenfassung:Genetic gain in potato is hampered by the heterozygous tetraploid genome of cultivated potato. Converting potato into a diploid inbred-line based F1-hybrid crop provides a promising route towards increased genetic gain. The introduction of a dominant S-locus inhibitor ( Sli ) gene into diploid potato germplasm allows efficient generation of self-fertilized seeds and thus the development of potato inbred lines. Little is known about the structure and function of the Sli locus. Here we describe the mapping of Sli to a 12.6 kb interval on chromosome 12 using a recombinant screen approach. One of two candidate genes present in this interval shows a unique sequence that is exclusively present in self-compatible lines. We describe an expression vector that converts self-incompatible genotypes into self-compatible and a CRISPR-Cas9 vector that converts SC genotypes into SI. The Sli gene encodes an F-box protein that is specifically expressed in pollen from self-compatible plants. A 533 bp insertion in the promotor of that gene leads to a gain of function mutation, which overcomes self-pollen rejection. The S-locus inhibitor ( Sli ) gene could allow potato breeding by facilitating production of diploid inbred lines. Here the authors show that Sli encodes an F-box protein with a promoter insertion enhancing expression in pollen can overcome pollen rejection in the styles of diploid potato.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-021-24267-6