Differential Effect of Viable Versus Necrotic Neutrophils on Mycobacterium tuberculosis Growth and Cytokine Induction in Whole Blood

Neutrophils exert both positive and negative influences on the host response to tuberculosis, but the mechanisms by which these differential effects are mediated are unknown. We studied the impact of live and dead neutrophils on the control of using a whole blood bioluminescence-based assay, and ass...

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Veröffentlicht in:	Frontiers in immunology 2018-04, Vol.9, p.903-903
Hauptverfasser:	Lowe, David M, Demaret, Julie, Bangani, Nonzwakazi, Nakiwala, Justine K, Goliath, Rene, Wilkinson, Katalin A, Wilkinson, Robert J, Martineau, Adrian R
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Assay - methods Cytokines - immunology Cytokines - metabolism Host-Pathogen Interactions - immunology Humans Immunology Life Sciences mycobacteria Mycobacterium tuberculosis - immunology necrosis Necrosis - immunology Necrosis - pathology neutrophil Neutrophils - immunology Neutrophils - pathology tuberculosis Tuberculosis - immunology Tuberculosis - microbiology viability
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Zusammenfassung:	Neutrophils exert both positive and negative influences on the host response to tuberculosis, but the mechanisms by which these differential effects are mediated are unknown. We studied the impact of live and dead neutrophils on the control of using a whole blood bioluminescence-based assay, and assayed supernatant cytokine concentrations using Luminex™ technology and ELISA. CD15+ granulocyte depletion from blood prior to infection with -lux impaired control of mycobacteria by 96 h, with a greater effect than depletion of CD4+, CD8+, or CD14+ cells (
ISSN:	1664-3224 1664-3224
DOI:	10.3389/fimmu.2018.00903