A REVIEW: MICROPROPAGATION OF PHALAENOPSIS sp FROM LEAF AND FLOWER STALK EXPLANTS

Abstract Phalaenopsis orchids are recognized as the most popular orchid genus in the world, especially in horticultural industry due to their large, colorful, and durable flowers as well as their wider adaptability to room conditions. The characteristics of seedling propagated by vegetative means are not uniform; therefore, propagation through tissue culture is desirable. Although the micro propagation of Phalaenopsis has shown very good development, but the wide spread of micro propagation still limited due some problems such as the exudation of phenolic compounds, the PGR concentration, the media used, somaclonal variation, the chosen explants, etc. This paper endeavor to include some important investigations based on the common explants used; leaf and flower stalk. Keywords: Micropropagation, Phalaenopsis, leaf explant, flower stalk ReferencesAnonymous. Orchid (Orchidaceae). Diakes tanggal 13 Januari 2013 dari http://www.rainforest-alliance.org/kids/species-profiles/orchid. Rainforest Alliance. 2002.Pillon, Y.; Chase, M. W.Taxonomic exaggeration and its effects on orchid conservation. Conservation Biology. 2007, 21, 263–265.Thengane, S. R.; Deodhar, S. R.; Bhosle, S. V.; Rawal, S. K. Direct somatic embryogenesis and plant regenaration in Garciniaindica Chois’. Current Science. 2006, 91(8), 1074-1078.Yuswanti, H.; Dharma, I. P.; Utama. ; Wiraatmaja, I. W. Mikropropagasi anggrek Phalaenopsis dengan menggunakan eksplan tangkai bunga. AGROTROP. 2015, 5(2): 161-166.Raynalta, E.; Sukma, D. Pengaruh komposisi media dalam perbanyakan protocorm like bodies, pertumbuhan plantlet, dan aklimatisasi Phalaenopsis amabilis. J. Hort. Indonesia. 2013, 4(3): 131-139.Kosir, P.; Skof, S.; Luthar, Z. Direct Shoot Regeneration from Nodes of Phalaenopsis of Orchids. Acta Agriculturae Slovenica. 2004, 83, 233–242.Arditti, J. R. ; Ernst. Micropropagation of Orchids. Wiley-Interscience. New York, 1993.Park, Y. S.;Kakuta, S.; Kano, A.; Okabe, M.Efficient propagation of protocorm-like bodies of Phalaenopsis in liquid medium. Plant Cell, Tissue and Organ Culture. 1996, 45, 79–85.Park, S. Y. ; Yeung, E. C.; Chakrabarty, D. ; Paek, K. Y. An efficient direct induction of protocorm-like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture. Plant Cell Reports. 2002, 21, 46–51.Zahara, M.; Datta, A.; Boonkorkaew, P. Effects of sucrose, carrot juice and culture media on growth and net CO2 exchange rate in Phalaenopsis hybrid ‘Pink’. ScientiaHorticulturae.