Propagation of Campanula bayerniana (Campanulaceae) by seeds as a reproduction model for the conservation of threatened small-seed plant species
The in vitro seed propagation of small seeded Campanula species often incurs complications in the manual handling of the tiny seed material from seed sterilisation to seedling transplantation in soil. The lack of a simple and effective protocol which minimises manual operations makes the propagation...
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Veröffentlicht in: | Botanica Serbica 2024-01, Vol.48 (2), p.223-229 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The in vitro seed propagation of small seeded Campanula species often incurs
complications in the manual handling of the tiny seed material from seed
sterilisation to seedling transplantation in soil. The lack of a simple and
effective protocol which minimises manual operations makes the propagation
of such species both challenging and inefficient. This study aimed to
develop a protocol for the seed reproduction of threatened Campanula and
other threatened small-seed plant species. The method was successfully
tested on Campanula bayerniana subsp. bayerniana. The saplings were
cultivated over a 5-month period through seed germination under in vitro
conditions followed by their transfer to containers with a soil mix. The
protocol employs small filter paper discs on which the seeds germinate and
continue their development in soil substrate until the full biodegradation
of the discs. The method involves surface treatment of the seeds with
potassium permanganate solution, thus avoiding additional washing phases and
direct contact with the seeds and seedlings, thereby preventing mechanical
damage. This also facilitates the handling of each seed separately,
minimising seed loss particularly when working with a small number of seeds.
This study is also the first known example of the ex situ propagation of
Campanula bayerniana. |
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ISSN: | 1821-2158 1821-2638 |
DOI: | 10.2298/BOTSERB2402223A |