Biological responses to heavy metal stress in the moss Leptodictyum riparium (Hedw.) Warnst

Leptodictyum riparium, a widely distributed aquatic moss, can both tolerate and accumulate very high concentrations of toxic heavy metals, with only slight apparent damage. Here we report the effects on photosynthetic yield, glutathione (GSH), phytochelatin (PCn) synthesis, nitrogen metabolism and cellular localization of molecules rich in SH groups in L. riparium exposed in vitro to heavy metals. We simulated the concentrations of Cu, Zn, Cd, Pb detected in Regi Lagni, Italy, one of the most contaminated freshwater sites in Southern Europe, in the laboratory to test how the moss responds to heavy metal contamination. There was a steady decrease of photosynthetic efficiency correlated with the heavy metal concentrations and ultrastructural organization. All PCn levels increased significantly as the concentration of heavy metals increased, while the GSH levels did not appear to be particularly affected. A significant increase of GDH and NADH-GOGAT activities increased with increasing heavy metal concentration. Immunoblotting analysis revealed an increase of the chl-GS2 while no significant increase was detected in the cyt-GS1. These results give insight into the molecular events underlying the metal-tolerance of the aquatic moss L. riparium exposed to environmental heavy metal concentrations. •The aquatic moss L. riparium was exposed in vitro to a mix of heavy metals.•The stressed conditions used simulate the metal concentrations detected in a strong contaminated freshwater site.•Morphological and biochemical changes were detected in L. riparium exposed to heavy metals at environmental concentrations.•Phytochelatins are synthetized in L. riparium in a dose-response manner.•Nitrogen metabolism is altered by heavy metal exposure.