Nitrogen and boron-doped reduced graphene oxide chemiresistive sensor for real-time monitoring dissolved oxygen in biological processes

•A chemiresistive sensor was developed for real time monitoring dissolved oxygen (DO) consumption in complex biological medium.•Nitrogen and Boron doped reduced graphene oxide conductive channel was formed on the interdigitated micro electrode arrays.•The influence of oxygen permeable membrane on the performance of N, B-HRGO chemiresistive DO sensor was shown.•Real-time DO changes during the Amycolatopsis methanolica bacterial fermentation process was perfomed. We developed a nitrogen and boron-doped reduced graphene oxide (N, B-HRGO) based chemiresistive sensor to measure dissolved oxygen (DO) in a complex biological medium. The N, B-HRGO modified interdigitated micro electrode arrays (IDE) constructed as a chemiresistor by the drop-cast method. A silicon based fluorinated oxygen permeable membrane protects the surface from the interference and provides a specificity to the sensor. The sensor responded to the DO concentration changes due to modulated surface charge carrier concentration by the adsorbed dissolved oxygen molecule (Oad). For DO concentration range 0–5 mg.L−1 there was nearly 80% change in response for the sensor with membrane. The resistance of the N, B-HRGO film was measured at different DO concentrations in KNO3 solution and during the growth of Amycalotopsis methanolica bacterial fermentation. The study showed that the sensor is sensitive to the oxygen present in the solution and can detect DO consumption in a complex fermentation medium. The effect of water and the electrolyte salt ions present in the electrolyte was studied in detail. It was observed that the adsorption of water molecule increases the sensor resistance, whereas the salt ions have negligible effect on the sensor response. Because of the simple electrode structure, this chemiresistive sensor can measure DO in the micro bioreactors with a volume of few microliters. The N, B-HRGO chemiresistive sensor can also be used for DO measurement in other bioprocess applications. [Display omitted]