Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TER...
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Veröffentlicht in: | Scientific reports 2017-06, Vol.7 (1), p.3810-7, Article 3810 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria’s lipid-bilayer membrane, which specifically enhances Gram negative bacteria. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-017-02212-2 |