Isolation and Characterization Identification of Edophytic Nitrogen-Fixing Bacteria from Peanut Nodules

This work was carried out to isolate and perform molecular identification and selection of endophytic nitrogen-fixing bacteria (ENFB) to be utilized as biofertilizer. In this research, nodulous samples of peanuts were collected from inside dyke areas, namely, Phuoc Hung of An Phu, An Giang, Vietnam. Ten colonies were isolated from nutrient agar plates containing YMA's medium. All isolates were rod shaped, Gram negative, and no spore creation. Biochemical tests indicated that they were obligate aerobes, catalase, oxidase, urea hydrolysis, well motile ability, and no nitrate reduction. The salt tolerance observed that most survived at 0.5% and 2% salinity (except subsp. strain LMG 2683), while at 4%, only 3 isolates ( strain CM44 strain IIWM-JS-07L, and strain KCa6) and at 5% only, 2 isolates survived, namely, strain IIWM-JS-07L and strain KCa6. The result showed that most of ten ENFB strains could adapt to the range of 25°C and 45°C (except subsp. strain LMG 2683 and Enterobacter mori strain cjy13 at 25°C). Out of ten isolates, three were finally selected for the next studies, which potentially have N-fixing ability and are utilized as biofertilizer in agricultural cultivation.