冷冻结晶法纯化制备高纯度磷脂酰乙醇胺Preparation of high-purity phosphatidylethanolamine by freezing crystallization

为解决工厂低磷脂酰胆碱（PC）含量大豆粉末磷脂的积压问题，以及获得高纯度的磷脂酰乙醇胺（PE），以用碱性乙醇从低PC含量大豆粉末磷脂中提取的粗PE产品为原料，通过冷冻结晶法纯化制备高纯度PE。在单因素实验的基础上，以PE含量为指标，通过响应面实验对冷冻结晶纯化条件进行了优化。结果表明，PE的最佳冷冻结晶纯化条件为：料液比1∶ 40，冷冻时间21.5 h，冷冻温度-25 ℃，乙醇体积分数94%，乙醇碱浓度为94%乙醇与25%氨水体积比100∶ 8。在最佳条件下，产品PE含量为76.56%，PE回收率为81.25%。因此，冷冻结晶法纯化工艺可用于制备高纯度PE。 To solve the backlog problem of soybean phospholipids with low phosphatidylcholine(PC)content in factories and to obtain high-purity phosphatidylethanolamine (PE), the crude PE product extracted from soybean phospholipids with low PC content by alkaline ethanol was used as the raw material to prepare high-purity PE by freezing crystallization. Based on the single factor experiment, with PE content as the indicator, the optimal freezing crystallization conditions of PE were determined by response surface methodology as follows: solid liquid ratio 1∶ 40, freezing time 21.5 h, freezing temperature -25 ℃,volume fraction of ethanol 94%, ethanol alkali concentration （volume ratio of 94% ethanol to 25% ammonia water） 100∶ 8. Under these conditions, the content and recovery rate of PE were 76.56% and 81.25%, respectively. Therefore, the freezing crystallization process can be used to prepare high-purity PE.