Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil

This study investigated the occurrence of rotavirus in porcine and Rattus norvegicus, at the same time, on a pig farm in the city of Jaguariúna, São Paulo, Brazil. Swine (n=21) and rat (n=6) fecal samples were analyzed by nested RT-PCR assay. Rotavirus occurred in seven porcine and two rat samples....

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Veröffentlicht in:	TheScientificWorld 2013-01, Vol.2013 (2013), p.1-4
Hauptverfasser:	Richtzenhain, L. J., Brandão, P. E., Ferrari, Karen Linares, Silva, Fernanda Dornellas F., Hora, Aline S., Tonietti, Paloma de Oliveira, Gregori, Fábio
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Husbandry Animals Brazil Farms Feces - virology Gastrointestinal Contents - virology Gene sequencing Group a rotavirus Health aspects Host-parasite relationships Phylogeny Porcine rotavirus Rats - virology Rattus norvegicus Rotavirus Rotavirus - classification Rotavirus - genetics Rotavirus - isolation & purification Rotaviruses Swine Swine - virology Virus research Zoological research
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Zusammenfassung:	This study investigated the occurrence of rotavirus in porcine and Rattus norvegicus, at the same time, on a pig farm in the city of Jaguariúna, São Paulo, Brazil. Swine (n=21) and rat (n=6) fecal samples were analyzed by nested RT-PCR assay. Rotavirus occurred in seven porcine and two rat samples. A total of three pig and one rat samples were further submitted to genetic sequencing. The partial NSP5 gene phylogeny showed that all strains were segregated in the genotype H1. These results point toward a cross-species transmission between rats and pigs on the surveyed farm and represent the first detection of rotavirus in Rattus norvegicus in Brazil.
ISSN:	2356-6140 1537-744X 1537-744X
DOI:	10.1155/2013/648406