RNAi-induced knockdown of white gene in the southern green stink bug (Nezara viridula L.)
The southern green stink bug (SGSB) Nezara viridula L. is one of the most common stink bug species in the United States and can cause significant yield loss in a variety of crops. A suitable marker for the assessment of gene-editing tools in SGSB has yet to be characterized. The white gene, first do...
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Veröffentlicht in: | Scientific reports 2022-06, Vol.12 (1), p.10396-10396, Article 10396 |
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Sprache: | eng |
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Zusammenfassung: | The southern green stink bug (SGSB)
Nezara viridula
L. is one of the most common stink bug species in the United States and can cause significant yield loss in a variety of crops. A suitable marker for the assessment of gene-editing tools in SGSB has yet to be characterized. The
white
gene, first documented in
Drosophila
, has been a useful target to assess the efficiency of introduced mutations in many species as it controls pigmentation processes and mutants display readily identifiable phenotypes. In this study we used the RNAi technique to investigate functions and phenotypes associated with the
white
ortholog in the SGSB and to validate
white
as a marker for genetic transformation in this species. This study revealed that
white
may be a suitable marker for germline transformation in the SGSB as
white
transcript knockdown was not lethal, did not impair embryo development and provided a distinguishable phenotype. Our results demonstrated that the
white
ortholog in SGSB is involved in the pathway for ommochrome synthesis and suggested additional functions of this gene such as in the integument composition, management of hemolymph compounds and riboflavin mobilization. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-022-14620-0 |