Quantification of panel of most potent antipsychotic medicines by high throughput UPLC method

•To identify overdose cases of antipsychotic drugs in the forensic laboratory.•The method can be applied to determine the chromatography purity of the said drug.•Simultaneous as well as single drug quantification is carried out with the method.•The method provides 0.06 ppm LOD with a 20–80 ppm linear range.•The method can be used to monitor the online reaction during manufacturing. The delegated work entails the simultaneous quantification and validation of a panel of common antipsychotic compounds in dosage form using the UPLC method. The antipsychotic medications used in the study were; Amisulpride, Risperidone, Paliperidone, Ziprasidone, Aripiprazole and Lurasidone. Acquity UPLC@BEH shield RP C18 (100 mm X 2.1 mm id, 1.7 m particle size) column was used to separate the samples with gradient elution consisting of 0.1% TFA and ACN with a PDA detector. The entire analysis took only 7 min with a 0.3 ml/min flow rate, 1 µl injection volume and a 45 °C column oven temperature. The current approach provides good resolution and low solvent consumption in a short amount of time. In most Quality Control laboratories, time-consuming methods and distinct mobile phases are used for different dosage forms of pharmaceuticals, however, the proposed method saves time and money by removing the need to change mobile phases. As a result, the method can be used in QC laboratories where time-consuming methods are used with different mobile-stationary phases. Antipsychotic drug analysis is mainly utilised in QC laboratory and forensic toxicology to monitor medication therapy or to explain the cause of intoxication in human performance and post-mortem situations.