Cholesterol transporter proteins in HepG2 cells can be modulated by phenolic compounds present in Opuntia ficus-indica aqueous solutions

[Display omitted] •Nopal’s decoction reduced NPC1L1, ABCG5/ABCG8 and ABCA1 in HepG2 cells.•Nopal’s decoction caused changes in the nucleic acid region of cells.•Piscidic, eucomic acids, isorhamnetin derivatives reduce cholesterol transporter proteins. Increased blood cholesterol is a risk factor for...

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Veröffentlicht in:Journal of functional foods 2020-01, Vol.64, p.103674, Article 103674
Hauptverfasser: Ressaissi, Asma, Attia, Nebil, Pacheco, Rita, Falé, Pedro Luis, Serralheiro, Maria Luísa M.
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Sprache:eng
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Zusammenfassung:[Display omitted] •Nopal’s decoction reduced NPC1L1, ABCG5/ABCG8 and ABCA1 in HepG2 cells.•Nopal’s decoction caused changes in the nucleic acid region of cells.•Piscidic, eucomic acids, isorhamnetin derivatives reduce cholesterol transporter proteins. Increased blood cholesterol is a risk factor for atherosclerotic cardiovascular disease. This study tested the hypothesis that phenolic compounds can modulate the level of cholesterol transporters including Niemann-Pick C1-like 1 (NPC1L1) and ATP-binding cassette transporters in liver cells. HepG2 cells, used as a model of hepatocytes, showed a decrease in the abundance of cholesterol transporters comparatively to the control when treated with the Opuntia ficus-indica’s cladodes decoction. The decrease was between 13–70%, 25–60%, 9–60% and 23–60% for NPC1L1, ABCA1, ABCG5 and ABCG8 transporters, respectively, when using between 0.15 and 0.35 mg/mL of decoction in the culture medium. FTIR analysis showed changes in the amount of RNA, which may be the cause of the decrease in the level of several proteins. These in vitro results pave the way to a molecular explanation for the decoction of cladodes effect on cholesterol levels as it reduced the membrane cholesterol transporter proteins, NPC1L1, ABCG5/ABCG8 and ABCA1, in HepG2 cells.
ISSN:1756-4646
2214-9414
DOI:10.1016/j.jff.2019.103674