Mechanisms involved in calcium oxalate endocytosis by Madin-Darby canine kidney cells

Calcium oxalate (CaOx) crystals adhere to and are internalized by tubular renal cells and it seems that this interaction is related (positively or negatively) to the appearance of urinary calculi. The present study analyzes a series of mechanisms possibly involved in CaOx uptake by Madin-Darby canin...

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Veröffentlicht in:	Brazilian journal of medical and biological research 2000-01, Vol.33 (1), p.111-118
Hauptverfasser:	Campos, A H, Schor, N
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals BIOLOGY calcium oxalate Calcium Oxalate - pharmacokinetics Cell Culture Techniques Crystallization DCK cells Dogs Endocytosis - drug effects Endocytosis - physiology Kidney - cytology Kidney - physiology mechanisms of endocytosis MEDICINE, RESEARCH & EXPERIMENTAL Microscopy, Polarization renal stone
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Zusammenfassung:	Calcium oxalate (CaOx) crystals adhere to and are internalized by tubular renal cells and it seems that this interaction is related (positively or negatively) to the appearance of urinary calculi. The present study analyzes a series of mechanisms possibly involved in CaOx uptake by Madin-Darby canine kidney (MDCK) cells. CaOx crystals were added to MDCK cell cultures and endocytosis was evaluated by polarized light microscopy. This process was inhibited by an increase in intracellular calcium by means of ionomycin (100 nM; N = 6; 43.9% inhibition; P
ISSN:	0100-879X 1414-431X 0100-879X 1414-431X
DOI:	10.1590/S0100-879X2000000100015