Metagenomic sequencing combined with flow cytometry facilitated a novel microbial risk assessment framework for bacterial pathogens in municipal wastewater without cultivation

Metagenomic sequencing combined with flow cytometry facilitated a novel microbial risk assessment framework for bacterial pathogens in municipal wastewater without cultivation

A workflow that combined metagenomic sequencing with flow cytometry was developed. The absolute abundance of pathogens was accurately estimated in mock communities and real samples. Metagenome‐assembled genomes binned from metagenomic data set is robust in phylogenetic analysis and virulence profili...

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Veröffentlicht in:iMeta 2023-02, Vol.2 (1), p.e77-n/a
Hauptverfasser: Fu, Songzhe, Wang, Rui, Xu, Zheng, Zhou, Huiwen, Qiu, Zhiguang, Shen, Lixin, Yang, Qian
Format: Artikel
Sprache:eng
Schlagworte:
Community
Efficiency
Estimates
Expected values
Flow cytometry
Pathogens
Risk assessment
Surveillance
Taxonomy
Virulence
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Beschreibung
Zusammenfassung:A workflow that combined metagenomic sequencing with flow cytometry was developed. The absolute abundance of pathogens was accurately estimated in mock communities and real samples. Metagenome‐assembled genomes binned from metagenomic data set is robust in phylogenetic analysis and virulence profiling.
ISSN:2770-596X
2770-5986
2770-596X
DOI:10.1002/imt2.77