The Neuroprotective Effect of Cannabinoid Receptor Agonist (WIN55,212-2) in Paraoxon Induced Neurotoxicity in PC12 Cells and N-methyl-D-aspartate Receptor Interaction

Objective: Considering that cannabinoids protect neurons against neurodegeneration, inthis study, the neuroprotective effect of WIN55,212-2 in paraoxon induced neurotoxicity inPC12 cells and the role of the N-methyl-D-aspartate (NMDA) receptor were evaluated.Materials and Methods: In this study PC12 cells were maintained in Dulbecco's modifiedeagle’s medium (DMEM+F12) culture medium supplemented with 10% fetal bovineserum. The cells were treated with paraoxon (200 μM) in the presence or absence ofWIN55,212-2 (0.1 μM), NMDA receptor agonist NMDA (100 μM), cannabinoid receptorantagonist AM251 and NMDA receptor antagonist MK801 (1 μM) at 15 minutes intervals.After 48 hours of exposure, cellular viability and protein expression of the CB1 receptorwere evaluated in PC12 cells.Results: Following the exposure of PC12 cells to paraoxon (200 μM), a reduction in cellsurvival and protein level of the CB1 receptor was observed (p