Progress in triacylglycerol isomer detection in milk lipids

In triacylglycerols (TAGs), position differences of fatty acids on the glycerol skeleton produce various TAG isomers. These TAG isomers have different pathways of digestion, absorption, and utilization in infants, thereby affecting TAG nutritional properties of TAGs. Here, we review the progress of research on methods for detecting TAG isomers, and identify direction and thought for improving these methods, including novel chromatographic combinations, perfect algorithm, and improved equipment. The ensuing optimization of these methods is expected to provide robust guarantee for the gradual improvement of milk-derived TAG isomer detection, and is an important prerequisite for infant formula to mimic the structured lipids of human milk. •Fatty acid location difference yield triacylglycerol (TAG) isomers with different functions.•The pros and cons of current methods for lactogenic TAG isomer detection summarized.•New chromatographic combinations might be improving TAG isomer analysis.