Structural basis of promoter recognition by Staphylococcus aureus RNA polymerase

Bacterial RNAP needs to form holoenzyme with σ factors to initiate transcription. While Staphylococcus aureus σ A controls housekeeping functions, S. aureus σ B regulates virulence, biofilm formation, persistence, cell internalization, membrane transport, and antimicrobial resistance. Besides the se...

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Veröffentlicht in:Nature communications 2024-06, Vol.15 (1), p.4850-9
Hauptverfasser: Yuan, Linggang, Liu, Qingyang, Xu, Liqiao, Wu, Bing, Feng, Yu
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Sprache:eng
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Zusammenfassung:Bacterial RNAP needs to form holoenzyme with σ factors to initiate transcription. While Staphylococcus aureus σ A controls housekeeping functions, S. aureus σ B regulates virulence, biofilm formation, persistence, cell internalization, membrane transport, and antimicrobial resistance. Besides the sequence difference, the spacers between the −35 element and −10 element of σ B regulated promoters are shorter than those of σ A regulated promoters. Therefore, how σ B recognizes and initiates transcription from target promoters can not be inferred from that of the well studied σ. Here, we report the cryo-EM structures of S. aureus RNAP-promoter open complexes comprising σ A and σ B , respectively. Structural analyses, in combination with biochemical experiments, reveal the structural basis for the promoter specificity of S. aureus transcription. Although the −10 element of σ A regulated promoters is recognized by domain σ A 2 as single-stranded DNA, the −10 element of σ B regulated promoters is co-recognized by domains σ B 2 and σ B 3 as double-stranded DNA, accounting for the short spacers of σ B regulated promoters. S. aureus RNAP is a validated target of antibiotics, and our structures pave the way for rational drug design targeting S. aureus RNAP. Here, Yuan, Liu, and Xu et al. report cryo-EM structures of Staphylococcus aureus RNAP-promoter open complexes, highlighting distinct interactions of σ A and σ B with their cognate promoters.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-024-49229-6