Molecular and Cytogenetic Identification of Wheat- Thinopyrum intermedium Double Substitution Line-Derived Progenies for Stripe Rust Resistance

(2n = 6x = 42, JJJ J StSt) has been hybridized extensively with common wheat and proven to be a valuable germplasm source for improving disease resistance and yield potential of wheat. A novel disease-resistant wheat- double substitution line X479, carrying 1St(1B) and 4St-4J (4B), was identified us...

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Veröffentlicht in:Plants (Basel) 2022-12, Vol.12 (1), p.28
Hauptverfasser: Li, Guangrong, Chen, Qiheng, Jiang, Wenxi, Zhang, Ahui, Yang, Ennian, Yang, Zujun
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Sprache:eng
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Zusammenfassung:(2n = 6x = 42, JJJ J StSt) has been hybridized extensively with common wheat and proven to be a valuable germplasm source for improving disease resistance and yield potential of wheat. A novel disease-resistant wheat- double substitution line X479, carrying 1St(1B) and 4St-4J (4B), was identified using multi-color non-denaturing fluorescence in situ hybridization (ND-FISH). With the aim of transferring -specific chromatin to wheat, a total of 573 plants from F and F progenies of X479 crossed with wheat cultivar MY11 were developed and characterized using sequential ND-FISH with multiple probes. Fifteen types of wheat- translocation chromosomes were preferentially transmitted in the progenies, and the homozygous wheat-1St, and wheat-4J L translocation lines were identified using ND-FISH, Oligo-FISH painting and CENH3 immunostaining. The wheat-4J L translocation lines exhibited high levels of resistance to stripe rust prevalent races in field screening. The gene for stripe rust resistance was found to be physically located on FL0-0.60 of the 4J L, using deletion lines and specific DNA markers. The new wheat- translocation lines can be exploited as useful germplasms for wheat improvement.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants12010028