Molecular and Cytogenetic Identification of Wheat- Thinopyrum intermedium Double Substitution Line-Derived Progenies for Stripe Rust Resistance
(2n = 6x = 42, JJJ J StSt) has been hybridized extensively with common wheat and proven to be a valuable germplasm source for improving disease resistance and yield potential of wheat. A novel disease-resistant wheat- double substitution line X479, carrying 1St(1B) and 4St-4J (4B), was identified us...
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Veröffentlicht in: | Plants (Basel) 2022-12, Vol.12 (1), p.28 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | (2n = 6x = 42, JJJ
J
StSt) has been hybridized extensively with common wheat and proven to be a valuable germplasm source for improving disease resistance and yield potential of wheat. A novel disease-resistant wheat-
double substitution line X479, carrying 1St(1B) and 4St-4J
(4B), was identified using multi-color non-denaturing fluorescence in situ hybridization (ND-FISH). With the aim of transferring
-specific chromatin to wheat, a total of 573 plants from F
and F
progenies of X479 crossed with wheat cultivar MY11 were developed and characterized using sequential ND-FISH with multiple probes. Fifteen types of wheat-
translocation chromosomes were preferentially transmitted in the progenies, and the homozygous wheat-1St, and wheat-4J
L translocation lines were identified using ND-FISH, Oligo-FISH painting and CENH3 immunostaining. The wheat-4J
L translocation lines exhibited high levels of resistance to stripe rust prevalent races in field screening. The gene for stripe rust resistance was found to be physically located on FL0-0.60 of the 4J
L, using deletion lines and specific DNA markers. The new wheat-
translocation lines can be exploited as useful germplasms for wheat improvement. |
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ISSN: | 2223-7747 2223-7747 |
DOI: | 10.3390/plants12010028 |