shRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus

Foot-and-mouth disease virus (FMDV) is one of the most important animal pathogens in the world. FMDV naturally infects swine, cattle, and other cloven-hoofed animals. FMD is not adequately controlled by vaccination. An alternative strategy is to develop swine that are genetically resistant to infect...

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Veröffentlicht in:	Scientific reports 2021-08, Vol.11 (1), p.16377-16377, Article 16377
Hauptverfasser:	Hu, Wenping, Zheng, Haixue, Li, Qiuyan, Wang, Yuhang, Liu, Xiangtao, Hu, Xiaoxiang, Liu, Wenjie, Liu, Shen, Chen, Zhisheng, Feng, Wenhai, Cai, Xuepeng, Li, Ning
Format:	Artikel
Sprache:	eng
Schlagworte:	631/61/17/1511 631/61/17/1998 Antibody response CD3 antigen CD4 antigen CD8 antigen Disease resistance Foot & mouth disease Humanities and Social Sciences Infections Infectious diseases multidisciplinary Nuclear transfer Ribonucleic acid RNA RNA-mediated interference Science Science (multidisciplinary) Somatic cell nuclear transfer Swine Vaccination Viremia γ-Interferon
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Zusammenfassung:	Foot-and-mouth disease virus (FMDV) is one of the most important animal pathogens in the world. FMDV naturally infects swine, cattle, and other cloven-hoofed animals. FMD is not adequately controlled by vaccination. An alternative strategy is to develop swine that are genetically resistant to infection. Here, we generated FMDV-specific shRNA transgenic cells targeting either nonstructural protein 2B or polymerase 3D of FMDV. The shRNA-positive transgenic cells displayed significantly lower viral production than that of the control cells after infection with FMDV ( P
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-021-95853-3