Strategies to determine the efficacy of multiple phytase use at low activities using Ross x Ross 708 male broilers from 0 to 14 d

Phytase inclusion provides broiler production advantages such as improved growth performance and better nutrient digestibility. These effects become heightened in response to more complete degradation of IP6 and its lower esters to prevent chelation of nutrients. Therefore, strategies for the complete removal of the total phytate pool are being investigated to maximize the benefits previously mentioned. The unique biochemical properties amongst commercial phytases may provide an opportunity for a more coordinated and efficacious approach to dephosphorylation if fed together. Therefore, 3 phytases (A, B, and C) were evaluated for potential synergistic action in breaking down on phytate using phytase inclusion strategies (PIS; A, B, C, AB, AC, BC, or ABC) at either 120 or 240 FTU/kg using 0–14 d chick performance, as well as tibia ash and ileal measurements (digestibility and IP esters). Positive and negative control (PC; NC) diets were formulated to contain 0.8% Ca and 0.4% nonphytate P (nPP) and 0.4% Ca and 0.20% nPP, respectively. Feeding NC + ABC at 240 FTU/kg led to improved BW gain and tibia ash percentage. Reduction of IP6/IP5 and increased IP isomers were found with NC + PIS treatments vs. PC, with benefits for PIS treatments at 240 FTU/kg. Supplementation of NC + B, C, and BC at 240 FTU/kg increased IP4 compared with PC. Digestibility data were inconsistent; however, improvements were demonstrated with feeding NC + B and BC at 240 FTU/kg. These data demonstrate a potential synergy with NC + ABC at 240 FTU/kg. Further research should investigate the effects of PIS with various diet specifications and at higher phytase activity levels.