Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia

Raw milk can be a source of food-borne disease transmission and a medium for spreading antibiotic-resistant bacteria. and are bacteria that have the pathogenic ability to attack host cells and are capable of harboring antibiotic-resistant genes. This study estimated the prevalence and antibiotic resistance of and isolated from raw milk in East Java, Indonesia. Two hundred and fifty raw milk samples were collected from five dairy farms in East Java. and were isolated using their respective selective media, whereas antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method. The methicillin-resistant (MRSA) was confirmed using the oxacillin resistance screen agar test, and extended-spectrum beta-lactamase (ESBL)-producing was determined using the double-disk synergy test. The presence of A and TEM genes were screened by the polymerase chain reaction method. Results indicated that the prevalence of was 138 (55.2%) and that was 176 (70.4%). Of the 138 isolated, 27 (19.6%) were MRSA, and among the 176 isolates identified, 3 (1.7%) were ESBL producers. The A gene was observed in 2 (7.4%) MRSA and all 3 (100%) ESBL-producing isolated harbored TEM genes. The presence of MRSA and ESBL-producing in raw milk is a serious public health threat, and public awareness should be raised about the dangers posed by these pathogenic organisms.