Histological, Laboratorial, and Clinical Benefits of an Optimized Maintenance Strategy of a Potential Organ Donor-A Rabbit Experimental Model

Most transplanted organs are obtained from brain-dead donors. Inflammation results in a higher rate of rejection. The objective of this animal model of brain death (BD) was to evaluate the effect of the progressive institution of volume expansion, norepinephrine, and combined hormone therapy on clin...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Life (Basel, Switzerland) Switzerland), 2023-06, Vol.13 (7), p.1439
Hauptverfasser: Tannous, Luana Alves, Westphal, Glauco Adrieno, Ioshii, Sergio Ossamu, de Lima Alves, Guilherme Naves, Pigatto, Raul Nishi, Pinto, Rafael Luiz, de Carvalho, Katherine Athayde Teixeira, Francisco, Júlio Cesar, Guarita-Souza, Luiz César
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Most transplanted organs are obtained from brain-dead donors. Inflammation results in a higher rate of rejection. The objective of this animal model of brain death (BD) was to evaluate the effect of the progressive institution of volume expansion, norepinephrine, and combined hormone therapy on clinical, laboratory, and histological aspects. Twenty rabbits were divided: A (control), B (induction of BD + infusion of crystalloid), C (BD + infusion of crystalloid and noradrenaline (NA)), and D (BD + infusion of crystalloid + vasopressin + levothyroxine + methylprednisolone + NA). The animals were monitored for four hours with consecutives analysis of vital signs and blood samples. The organs were evaluated by a pathologist. In Group D, we observed fewer number and lesser volume of infusions ( = 0.032/0.014) when compared with groups B and C. Mean arterial pressure levels were higher in group D when compared with group B ( = 0.008). Group D had better glycemic control when compared with group C ( = 0.016). Sodium values were elevated in group B in relation to groups C and D ( = 0.021). In Group D, the organ perfusion was better. The optimized strategy of management of BD animals is associated with better hemodynamic, glycemic, and natremia control, besides reducing early signs of ischemia.
ISSN:2075-1729
2075-1729
DOI:10.3390/life13071439