A Python script to merge Sanger sequences

A Python script to merge Sanger sequences

Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The r...

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Veröffentlicht in:PeerJ (San Francisco, CA) CA), 2021-04, Vol.9, p.e11354-e11354, Article e11354
Hauptverfasser: Chen, Cen, Lu, Bingguo, Huang, Xiaofang, Bi, Chuyun, Zhao, Lili, Hu, Yunzhuo, Chen, Xuanyang, Lin, Shiqiang, Huang, Kai
Format: Artikel
Sprache:eng
Schlagworte:
Algorithms
Bioinformatics
Cloning
Computational Science
DNA sequencing
Genes
Genetics
Genomes
Genomics
Merge
Molecular Biology
Nucleotide sequence
Nucleotide sequencing
Python script
Sanger sequencing
Software
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Zusammenfassung:Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.11354