The importance of matrix-assisted laser desorption ionization–time of flight mass spectrometry for correct identification of Clostridium difficile isolated from chromID C. difficile chromogenic agar

Abstract The clinical workflow of using chromogenic agar and matrix-assisted laser desorption ionization time-of-fight mass spectrometry (MALDI-TOF MS) for Clostridium difficile identification was evaluated. The addition of MALDI-TOF MS identification after the chromID C. difficile chromogenic agar...

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Veröffentlicht in:	Journal of microbiology, immunology and infection immunology and infection, 2017-10, Vol.50 (5), p.723-726
Hauptverfasser:	Chen, Jonathan H.K, Cheng, Vincent C.C, Wong, Oi-Ying, Wong, Sally C.Y, So, Simon Y.C, Yam, Wing-Cheong, Yuen, Kwok-Yung
Format:	Artikel
Sprache:	eng
Schlagworte:	Agar Bacteriological Techniques - methods chromogenic agar Chromogenic Compounds - chemistry Clostridium difficile Clostridium difficile - classification Clostridium difficile - genetics Clostridium difficile - growth & development Clostridium difficile - isolation & purification Clostridium Infections - diagnosis Clostridium Infections - microbiology Color Culture Media - chemistry Humans Infectious Disease matrix-assisted laser desorption ionization–time of flight Medical Education Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Zusammenfassung:	Abstract The clinical workflow of using chromogenic agar and matrix-assisted laser desorption ionization time-of-fight mass spectrometry (MALDI-TOF MS) for Clostridium difficile identification was evaluated. The addition of MALDI-TOF MS identification after the chromID C. difficile chromogenic agar culture could significantly improve the diagnostic accuracy of C. difficile.
ISSN:	1684-1182 1995-9133
DOI:	10.1016/j.jmii.2015.12.002