In vitro evaluation of influence of salivary contamination on the dentin bond strength of one-bottle adhesive systems

To evaluate the effect of salivary contamination on the bond strength of one-bottle adhesive systems - (the V generation) at various stages during the bonding procedure and to investigate the effect of the contaminant removing treatments on the recovery of bond strengths. In this study the V generation one-bottle system - (Adper Single Bond) was tested. Fifty caries-free human molars with flat dentin surfaces were randomly divided into five groups of ten teeth each: Group I had 15 second etching with 35% Ortho Phosphoric acid, 15 second rinse and blot dried (Uncontaminated); Group II contaminated and blot dried; Group III contaminated and completely dried; Group IV contaminated, washed, blot dried; Group V contaminated, retched washed, and blot dried. The bonding agent was applied and resin composite (Z-100 3M ESPE) was bonded to the treated surfaces using the Teflon mold. The specimens in each group were then subjected to shear bond strength testing in an Instron Universal testing machine at a crosshead speed of 1 mm / minute and the data were subjected to one way ANOVA for comparison among the groups (P