Study on the anti-inflammatory activity of chaga mushroom aqueous extract-alcohol precipitate

[Abstract]: Objective: To extract the active ingredient from chaga mushroom (aqueous extract-alcohol precipitates) to study its safety and anti-inflammatory activity. Methods: The aqueous extraction-alcohol precipitation method was used to extract the active ingredient of chaga mushroom for mass fraction analysis of polysaccharides. Zebrafish embryos were used for the acute toxicity study and the median lethal dose (LD50) was used to assess the safety of the aqueous extract-alcohol precipitates. Physical methods were used to amputate the caudal fins of zebrafish and induce inflammation, and this model was used to study the anti-inflammatory activity. Results: The LD50 of chaga mushroom aqueous extract-alcohol precipitate measured in the acute toxicity study was 6.888 mg/mL. The anti-inflammatory experimental results show that chaga mushroom aqueous extract-alcohol precipitate inhibit the aggregation of neutrophils and macrophages at inflammation sites, differences were statistically significant (P < 0.05). Conclusion: Chaga mushroom aqueous extract-alcohol precipitates exhibits good anti-inflammatory effects within the safe concentrations.