Generation of two induced pluripotent stem cell lines (RCMGi005-A/B) from human skin fibroblasts of a cystic fibrosis patient with homozygous F508del mutation in CFTR gene

Generation of two induced pluripotent stem cell lines (RCMGi005-A/B) from human skin fibroblasts of a cystic fibrosis patient with homozygous F508del mutation in CFTR gene

Induced pluripotent stem cells (iPSCs) was successfully generated from skin fibroblast obtained from patient with cystic fibrosis by using non-integrating, viral CytoTune™-iPS 2.0 Sendai Reprogramming Kit, which contain three vectors preparation: polycistronic Klf4–Oct3/4–Sox2, cMyc, and Klf4. Creat...

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Veröffentlicht in:Stem cell research 2022-10, Vol.64, p.102896-102896, Article 102896
Hauptverfasser: Panchuk, IO, Kondrateva, EV, Demchenko, AG, Grigorieva, OV, Erofeeva, AV, Amelina, EL, Tabakov, VYu, Orlova, MD, Voronina, ES, Pozhitnova, VO, Lavrov, AV, Smirnikhina, SA, Kutsev, SI
Format: Artikel
Sprache:eng
Schlagworte:
Cell reprograming
cystic fibrosis (CF)
Induced pluripotent stem cells (iPSC)
Pluripotency
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Zusammenfassung:Induced pluripotent stem cells (iPSCs) was successfully generated from skin fibroblast obtained from patient with cystic fibrosis by using non-integrating, viral CytoTune™-iPS 2.0 Sendai Reprogramming Kit, which contain three vectors preparation: polycistronic Klf4–Oct3/4–Sox2, cMyc, and Klf4. Created iPSC lines showed a normal karyotype, expressed pluripotency markers and demonstrated the potential to differentiate into three germ layers in spontaneous differentiation assay.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.102896