Evaluation of Cassia tora Linn. against Oxidative Stress-induced DNA and Cell Membrane Damage

The present study aims to evaluate antioxidants and protective role of Linn. against oxidative stress-induced DNA and cell membrane damage. The total and profiles of flavonoids were identified and quantified through reversed-phase high-performance liquid chromatography. antioxidant activity was determined using standard antioxidant assays. The protective role of extracts against oxidative stress-induced DNA and cell membrane damage was examined by electrophoretic and scanning electron microscopic studies, respectively. The total flavonoid content of CtEA was 106.8 ± 2.8 mg/g d.w.QE, CtME was 72.4 ± 1.12 mg/g d.w.QE, and CtWE was 30.4 ± 0.8 mg/g d.w.QE. The concentration of flavonoids present in CtEA in decreasing order: quercetin >kaempferol >epicatechin; in CtME: quercetin >rutin >kaempferol; whereas, in CtWE: quercetin >rutin >kaempferol. The CtEA inhibited free radical-induced red blood cell hemolysis and cell membrane morphology better than CtME as confirmed by a scanning electron micrograph. CtEA also showed better protection than CtME and CtWE against free radical-induced DNA damage as confirmed by electrophoresis. contains flavonoids and inhibits oxidative stress and can be used for many health benefits and pharmacotherapy.