Identifying the intervention mechanisms of o-methoxycinnamic acid in hyperuricemia model by using UPLC-Q-TOF/MS spectroscopy metabonomic approach

[Display omitted] •OMA decreased the uric acid content and increased its median lethality time in nematode models.•OMA has anti-hyperuricemic activity.•OMA mainly affected the purine metabolic pathway and amino acid related metabolic pathway of nematodes.•Amino acids such as phenylalanine, alanine, tryptophan, arginine, proline and histidine can be used as potential diagnostic markers. The anti-hyperuricemic activity of o-methoxycinnamic acid (OMA) was investigated in the Caenorhabditis elegans (C. elegans) model, and its mode of action and biomarkers were studied using metabolomics techniques. First, a hyperuricemic C. elegans model was established to study the anti-hyperuricemic activity of OMA by altering uric acid levels in the nematodes. Subsequently, UPLC-Q-TOF/MS was used to investigate the effect of OMA on C. elegans endogenous metabolites, and differential metabolite analysis was used to identify OMA action pathways and biomarkers. The results showed that uric acid level increased by 12.0% (P