Synergistic Antimicrobial Effect of Lactiplantibacillus plantarum and Lawsonia inermis Against Staphylococcus aureus

The developed resistance of pathogenic microorganisms towards the currently used antimicrobial agents requires the fast search for newer potent antimicrobials. One of the most important ways to combat the previously mentioned disaster is the use of natural alternatives like medicinal plants. Our study aimed to estimate the anti-inflammatory property, and antibacterial effects of probiotics and ethanol extracts of leaves against when they were used separately or collectively as synergism. Experimentally induced infected wound model in mice was created and divided into 10 groups then treated for two days by and individually or in combination, followed by biochemical assays. The antibacterial, anti-inflammatory, and wound healing activity were evaluated through histopathological sections taken before and after treatment. Our results revealed that and mixture could inhibit growth of and decrease the minimal inhibitory concentration (MIC) of to 2 mg/mL. The mixture decreased level of both interleukin 6 (IL-6) and interferon-alpha (TNF-α) to a level near the normal uninfected group. Histopathological study showed that animals treated with both and had achieved almost 90% healing. These results suggest that and mixture has synergistic effect on healing of infected wound.