Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method

The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a significant impact on global health. To address the urgent need for plasmids containing SARS-CoV-2 sequences in research, we have developed a high-throughput FastCloning platform for the construction of associated plasmids. Our platform uses a FastCloning method to construct a plasmid library from 29 ORFs of the virus and 20 commonly used vectors in the lab. The library contains 536 recombinant vectors, with a highly positive clone success rate of 92.4%. Our study provides a rapid and efficient approach to constructing a large plasmid library for SARS-CoV-2 research. •a high-throughput FastCloning method for constructing a SARS-CoV-2 plasmid library.•Our FastCloning platform produced 536 SARS-CoV-2 gene-associated expression vectors with a 92.4% success rate, offering an efficient resource to accelerate COVID-19 research.•The plasmid library offers a valuable resource for developing new COVID-19 treatments and vaccines, as well as for further research on the virus and its potential as a therapeutic target.