Effect of copper sulfate on liver damage induced by nano-zinc oxide in Cyprinus carpio

The effects of Copper Sulfate (CuSO4) on repaired the histopthological changes on liver of Cyprinus carpio exposed to nano zinc oxide (N-ZnO) was determined in this study.Fish weight 150±10 g divided in to four groups.Fish in first group kept in dechlorinated water while the fish in second group exposed to copper sulfate CuSO4 for 1 hour and in third group the fish was exposed to sub lethal concentration of N-ZnO (30ppm) for 7 days while the fish in fourth group exposed to sub lethal concentration of N-ZnO (30ppm) with CuSO4 (0.3mg\L) for 1hour and then exposed continuously to N-ZnO for 7days. The microscopic examination for the liver done through 24 hour and 7 days from the experiment, which revealed vacuolar degeneration in hepatic cells in fish exposed to N-ZnO for 24 hour also there were dilatation of sinusoid and infiltration of inflammatory cells in liver tissue of fish exposed to to N-ZnO with CuSO4 for 1 hour, there were slight hemorrhage in hepatic tissue and enlargement of hepatic cell in both groups exposed to N-ZnO and CuSO4 for 1 and 24 hour, while the microscopically examination of liver in fish exposed to N-ZnO for 7 days revealed sever pathological lesions characterized by hyperatrophy and necrosis in pancreatic tissue. This study concluded that N-ZnO can cause oxidative stress and usage of CuSO4 at 0.3 mg/L can decrease the toxic effects of N-ZnO liver tissue.