Male Sterility of an AHAS -Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L
Tribenuron-methyl (TBM), an acetohydroxyacid synthase (AHAS)-inhibiting herbicide, can be used as an efficient chemical hybridization agent to induce male sterility for practical utilization of heterosis in rapeseed ( L.). Utilization of rapeseed mutants harboring herbicide-resistant AHAS alleles as...
Gespeichert in:
Veröffentlicht in: | Frontiers in plant science 2018-07, Vol.9, p.1014-1014 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Tribenuron-methyl (TBM), an acetohydroxyacid synthase (AHAS)-inhibiting herbicide, can be used as an efficient chemical hybridization agent to induce male sterility for practical utilization of heterosis in rapeseed (
L.). Utilization of rapeseed mutants harboring herbicide-resistant AHAS alleles as the male parent can simplify the hybrid seed production protocol. Here we characterized a novel TBM-resistant mutant
derived from an elite rapeseed variety, Zhongshuang No. 9 (
), by ethyl methyl sulfonate mutagenesis. Comparative analysis of three
genes (
, and
) between the mutant
and
identified a C-to-T transition at 544 from the translation start site in
in
(This resistant allele is referred to as
), which resulted in a substitution of proline with serine at 182 in BnAHAS1. Both
and
plants could be induced complete male sterility under TBM treatment (with 0.10 and 20 mg⋅L
of TBM, respectively). The relationship between TBM-induced male sterility (
) and the relative AHAS activity of inflorescences (
) could be described as a modified logistic function,
= 100-
/(1+
) for the both genotypes, although the obtained constants
, and
were different in the functions of
and
. Transgenic
plants expressing
exhibited a higher TBM resistance of male reproductive organ than wild type, which confirmed that the Pro-182-Ser substitution in BnAHAS1 was responsible for higher TBM-resistance of male reproductive organs. Taken together, our findings provide a novel valuable rapeseed mutant for hybrid breeding by chemical hybridization agents and support the hypothesis that AHAS should be the target of the AHAS-inhibiting herbicide TBM when it is used as chemical hybridization agent in rapeseed. |
---|---|
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2018.01014 |