Increased expression of miR-320b in blood plasma of patients in response to SARS-CoV-2 infection

Coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Recent research has demonstrated how epigenetic mechanisms regulate the host–virus interactions in COVID-19. It has also shown that microRNAs (miRNAs) are one of the three fundamental mecha...

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Veröffentlicht in:Scientific reports 2024-06, Vol.14 (1), p.13702-10
Hauptverfasser: de Souza Nicoletti, Aline, Berlofa Visacri, Marília, Regina da Silva Correa da Ronda, Carla, Tiemi Siguemoto, Julia, Motta Neri, Carolini, Nogueira de Souza, Rafael, de Souza Ventura, Deise, Eguti, Adriana, Ferreira de Souza Silva, Lilian, Wesley Perroud Junior, Mauricio, Buosi, Keini, Jalalizadeh, Mehrsa, Dionato, Franciele, Dal Col, Luciana, Giacomelli, Cristiane, Leme, Patrícia, Oliveira Reis, Leonardo, Augusto dos Santos, Luiz, Durán, Nelson, José Fávaro, Wagner, Luiz da Costa, José, Dagli-Hernandez, Carolina, Moriel, Patricia, de Carvalho Pincinato, Eder
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Sprache:eng
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Zusammenfassung:Coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Recent research has demonstrated how epigenetic mechanisms regulate the host–virus interactions in COVID-19. It has also shown that microRNAs (miRNAs) are one of the three fundamental mechanisms of the epigenetic regulation of gene expression and play an important role in viral infections. A pilot study published by our research group identified, through next-generation sequencing (NGS), that miR-4433b-5p, miR-320b, and miR-16–2-3p are differentially expressed between patients with COVID-19 and controls. Thus, the objectives of this study were to validate the expression of these miRNAs using quantitative real-time polymerase chain reaction (qRT-PCR) and to perform in silico analyses. Patients with COVID-19 (n = 90) and healthy volunteers (n = 40) were recruited. MiRNAs were extracted from plasma samples and validated using qRT-PCR. In addition, in silico analyses were performed using mirPath v.3 software. MiR-320b was the only miRNA upregulated in the case group com-pared to the control group. The in silico analyses indicated the role of miR-320b in the regulation of the KITLG gene and consequently in the inflammatory process. This study confirmed that miR-320b can distinguish patients with COVID-19 from control participants; however, further research is needed to determine whether this miRNA can be used as a target or a biomarker.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-64325-9