Non-coding Class Switch Recombination-Related Transcription in Human Normal and Pathological Immune Responses

Antibody class switch recombination (CSR) to IgG, IgA, or IgE is a hallmark of adaptive immunity, allowing antibody function diversification beyond IgM. CSR involves a deletion of the IgM/IgD constant region genes placing a new acceptor Constant gene, downstream of the VDJ exon. CSR depends on non-coding (CSRnc) transcription of donor I and acceptor I exons, located 5' upstream of each C coding gene. Although, our knowledge of the role of CSRnc transcription has advanced greatly, its extension and importance in healthy and diseased humans is scarce. We analyzed CSRnc transcription in 70,603 publicly available RNA-seq samples, including GTEx, TCGA, and the Sequence Read Archive using , an online resource consisting of normalized RNA-seq gene and exon counts, as well as, coverage files that can be programmatically accessed through R. CSRnc transcription was validated with a qRT-PCR assay for I , I , and I in humans in response to vaccination. We mapped I transcription for the human IGH locus, including the less understood gene. CSRnc transcription was restricted to B cells and is widely distributed in normal adult tissues, but predominant in blood, spleen, MALT-containing tissues, visceral adipose tissue and some so-called "immune privileged" tissues. However, significant I expression was found even in non-lymphoid fetal tissues. CSRnc expression in cancer tissues mimicked the expression of their normal counterparts, with notable pattern changes in some common cancer subsets. CSRnc transcription in tumors appears to result from tumor infiltration by B cells, since CSRnc transcription was not detected in corresponding tumor-derived immortal cell lines. Additionally, significantly increased I transcription in ileal mucosa in Crohn's disease with ulceration was found. In conclusion, CSRnc transcription occurs in multiple anatomical locations beyond classical secondary lymphoid organs, representing a potentially useful marker of effector B cell responses in normal and pathological immune responses. The pattern of I exon expression may reveal clues of the local immune response (i.e., cytokine milieu) in health and disease. This is a great example of how the public data can be used to further our understanding of transcription, including regions outside the known transcriptome.