Random Amplified Polymorphic DNA Analysis of DNA Extracted from Trichuris trichiura (Linnaeus, 1771) Eggs and its Prospective Application to Paleoparasitological Studies

Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and D...

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Veröffentlicht in:	Memórias do Instituto Oswaldo Cruz 2003-01, Vol.98 (suppl 1), p.59-62
Hauptverfasser:	Martinez, Elaine Machado, Correia, Jorge Antonio Santos, Villela, Erika Verissimo, Duarte, Antonio Nascimento, Ferreira, Luiz Fernando, Bello, Alexandre Ribeiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence DNA, Helminth - genetics DNA, Helminth - isolation & purification Electrophoresis, Agar Gel Genetic Markers Humans Molecular Sequence Data Ovum paleoparasitology PARASITOLOGY random amplified polymorphic DNA analysis random amplified polymorphic DNA analysis - Trichuris trichiura - paleoparasitology Random Amplified Polymorphic DNA Technique Trichuris - genetics Trichuris trichiura TROPICAL MEDICINE
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Zusammenfassung:	Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and DNA sequencing of some of these bands from agarose gels, might help in establishing T. trichiura specific genetic markers, not available yet, and an important step to design primers to be used in molecular diagnosis approaches.
ISSN:	1678-8060 0074-0276 0074-0276 1678-8060
DOI:	10.1590/s0074-02762003000900011