Generation of human induced pluripotent stem cell line from Alzheimer’s disease patient with PSEN2 N141I mutation using integration-free non-viral method

Generation of human induced pluripotent stem cell line from Alzheimer’s disease patient with PSEN2 N141I mutation using integration-free non-viral method

Induced pluripotent stem cell (iPSC) line HUi001-A was reprogrammed from skin fibroblasts via non-integrating, virus free self-replicating RNA. Skin fibroblasts from an 81-year-old female Caucasian familial Alzheimer’s disease patient of Volga German family carrying N141I mutation in the PSEN2 gene...

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Veröffentlicht in:Stem cell research 2020-08, Vol.47, p.101892-101892, Article 101892
Hauptverfasser: Murti, Harry, Pieknell, Kelvin, Bachtiar, Indra, Sulistio, Yanuar Alan, Lee, Sang-Hun
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Sprache:eng
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Zusammenfassung:Induced pluripotent stem cell (iPSC) line HUi001-A was reprogrammed from skin fibroblasts via non-integrating, virus free self-replicating RNA. Skin fibroblasts from an 81-year-old female Caucasian familial Alzheimer’s disease patient of Volga German family carrying N141I mutation in the PSEN2 gene (familial AD4, clinical summary confirmed Alzheimer’s disease) was obtained from the Coriell Institute (AG09908). Generated iPSCs were characterized and pluripotency was confirmed.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2020.101892