Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry

Time-domain measurement of the fluorescence lifetime in flow cytometry offers a complementary method to traditional flow cytometry, especially for the analysis of cells and biomolecules. Short measurement times due to high throughputs (cells/s) make a suited detector mandatory for a sufficient detec...

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Veröffentlicht in:Current directions in biomedical engineering 2023-09, Vol.9 (1), p.363-366
Hauptverfasser: Rottmann, Jonas, Schierbaum, Nicolas, Netaev, Alexander, Ligges, Manuel, Seidl, Karsten
Format: Artikel
Sprache:eng
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Zusammenfassung:Time-domain measurement of the fluorescence lifetime in flow cytometry offers a complementary method to traditional flow cytometry, especially for the analysis of cells and biomolecules. Short measurement times due to high throughputs (cells/s) make a suited detector mandatory for a sufficient detection of the fluorescence signal and therefore the application of this method. We summarize the requirements for a suited detector for time resolved flow cytometry (TRFC) and present a new developed SPAD array detector with 100 pixels and 340 ns time between measurement windows, allowing a high-throughput of up to 60,000 cells/s. The functionality of the detector is shown with the measurement of a 22.2 ns laser pulse, making it applicable for an improved TRFC.
ISSN:2364-5504
2364-5504
DOI:10.1515/cdbme-2023-1091