Differential response of fasting and postprandial angiopoietin-like proteins 3, -4, and -8 to cottonseed oil versus olive oil

Timeline of study visits and measurements. Study visits and measurements. CSO; Cotton seed oil; OO; Olive oil; RMR; Resting metabolic rate. [Display omitted] •Cottonseed oil (CSO) contains a bioactive lipid, dihydrosterculic acid.•Dihydrosterculic acid improves lipid and lipoprotein levels.•Postprandial ANGPTL3, -4 and -8 levels may mediate CSOs effects.•Olive oil and CSO display differential effects on fasting and postprandial ANGPTLs. Angiopoietin-like proteins (ANGPTL) 3, -4 and -8 regulate lipid metabolism by altering lipoprotein lipase activity, but it is not known how cottonseed oil (CSO) differentially affects postprandial responses of ANGPTL’s in humans. We compared the effect of a diet rich in either CSO or olive oil (OO) on fasting and postprandial ANGPTL’s. Fifteen males completed two feeding trials in random order: 1) a CSO-rich diet and 2) an OO-rich diet with fasting and postprandial blood samples collected pre-diet at visit 1 (v1) followed by a 5-day feeding trial and a post-diet visit (v2) identical to v1 and a 2–4-week washout period. Fasting ANGPTL4 and ANGPTL8 decreased from v1 to v2 after the CSO diet and increased with the OO diet. Postprandial ANGPTL3, -4, and -8 were lower following the CSO meal. The lipid-lowering effects of CSO may be mediated by reduced fasting and postprandial ANGPTL3, -4 and -8 responses.