The performance of homopolymer detection using dichromatic and tetrachromatic fluorogenic next-generation sequencing platforms

Homopolymer (HP) sequencing is error-prone in next-generation sequencing (NGS) assays, and may induce false insertion/deletions and substitutions. This study aimed to evaluate the performance of dichromatic and tetrachromatic fluorogenic NGS platforms when sequencing homopolymeric regions. A HP-cont...

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Veröffentlicht in:BMC genomics 2024-05, Vol.25 (1), p.542-10, Article 542
Hauptverfasser: Chen, HuiJuan, Wang, Bing, Cai, LiLi, Zhang, YiRan, Shu, YingShuang, Liu, Wen, Leng, Xue, Zhai, JinCheng, Niu, BeiFang, Zhou, QiMing, Cao, ShuNan
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Sprache:eng
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Zusammenfassung:Homopolymer (HP) sequencing is error-prone in next-generation sequencing (NGS) assays, and may induce false insertion/deletions and substitutions. This study aimed to evaluate the performance of dichromatic and tetrachromatic fluorogenic NGS platforms when sequencing homopolymeric regions. A HP-containing plasmid was constructed and diluted to serial frequencies (3%, 10%, 30%, 60%) to determine the performance of an MGISEQ-2000, MGISEQ-200, and NextSeq 2000 in HP sequencing. An evident negative correlation was observed between the detected frequencies of four nucleotide HPs and the HP length. Significantly decreased rates (P 
ISSN:1471-2164
1471-2164
DOI:10.1186/s12864-024-10474-0